time spent

Lecture (03/11)

<aside> <img src="/icons/slide_green.svg" alt="/icons/slide_green.svg" width="40px" /> Lecture slides: • Chris Mason, "A 500-year plan for engineering life on Earth and beyond" • Lisa Riedmayr, “Genetic Medicines”

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<aside> <img src="/icons/video-camera_yellow.svg" alt="/icons/video-camera_yellow.svg" width="40px" /> Lecture Recording

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Recitation (03/12)

<aside> <img src="/icons/slide_green.svg" alt="/icons/slide_green.svg" width="40px" /> Recitation slides will be shared here after the class!

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<aside> <img src="/icons/video-camera_yellow.svg" alt="/icons/video-camera_yellow.svg" width="40px" /> Recording will be updated here after the class!

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Homework

<aside> <img src="/icons/exclamation-mark_orange.svg" alt="/icons/exclamation-mark_orange.svg" width="40px" /> These homework questions are based on the Gibson Assembly Lab! Mandatory for both CL and MIT/Harvard students.

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<aside> <img src="/icons/push-pin_green.svg" alt="/icons/push-pin_green.svg" width="40px" /> Key Links: https://docs.google.com/document/d/1_aSV7w8iRYc3EDmbueJ_hSEGy_jHLDfxT2wAezEtC4c/edit?tab=t.0#heading=h.a157u2dx9dhb

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What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose?

I find this website useful

[Phusion® High-Fidelity PCR Master Mix with HF Buffer | NEB](https://www.neb.com/en/products/m0531-phusion-high-fidelity-pcr-master-mix-with-hf-buffer#:~:text=Phusion High-Fidelity PCR Master Mix with HF Buffer,is the addition of template%2C primers and water.)